{"product_id":"benchmark-scientific-accuris-high-fidelity-pcr","title":"Benchmark Scientific Accuris™ High Fidelity PCR","description":"\u003ch2\u003e\u003cstrong\u003eBenchmark Scientific Accuris™ High Fidelity PCR, Optimized Buffers\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFor applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options.  Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUp to 100X higher fidelity than Taq polymerase.\u003c\/li\u003e\n\u003cli\u003eCrude DNA sample will not inhibit amplification.\u003c\/li\u003e\n\u003cli\u003eIdeal for cloning, mutagenesis, sequencing and microarrays.\u003c\/li\u003e\n\u003cli\u003eOptions for blunt end or A-overhang products.\u003c\/li\u003e\n\u003cli\u003eOptimized buffers include PCR enhancers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cstrong\u003eAccuris High Fidelty Polymerase (2 tube formulation):\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLeaves a blunt end for blunt end vector cloning\u003c\/li\u003e\n\u003cli\u003e50x fidelity of native Taq\u003c\/li\u003e\n\u003cli\u003eIdeal for complex targets up to 10kb\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFor applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit.  Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples.\u003c\/p\u003e\n\u003cp\u003eA 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications.  The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003eAccuris High Fidelity Master Mix:\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLeaves a blunt end for blunt end vector cloning\u003c\/li\u003e\n\u003cli\u003e100x fidelity of native Taq\u003c\/li\u003e\n\u003cli\u003eIdeal for shorter, less complex targets\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eA 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.\u003c\/p\u003e\n\u003cp\u003eThe pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003eAccuris High Fidelity, Hot Start Master Mix:\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eLeaves an A-overhang for TA cloning\u003c\/li\u003e\n\u003cli\u003e10x fidelity of native Taq\u003c\/li\u003e\n\u003cli\u003eIdeal for difficult, high GC content sequences up to 10kb\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAccuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance.\u003c\/p\u003e\n\u003cp\u003eThe pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.\u003c\/p\u003e","brand":"Benchmark Scientific","offers":[{"title":"Accuris High Fidelity DNA Polymerase 200 units","offer_id":52179873857874,"sku":"PR1000-HF-200","price":148.65,"currency_code":"USD","in_stock":true},{"title":"Accuris High Fidelity DNA Polymerase 1000 units","offer_id":52179873890642,"sku":"PR1000-HF-1000","price":553.78,"currency_code":"USD","in_stock":true},{"title":"Accuris High Fidelity Master Mix 200 rxns","offer_id":52179873923410,"sku":"PR1001-HF-200","price":160.55,"currency_code":"USD","in_stock":true},{"title":"Accuris High Fidelity Master Mix 500 rxns","offer_id":52179873956178,"sku":"PR1001-HF-500","price":356.61,"currency_code":"USD","in_stock":true},{"title":"Accuris High Fidelity Hot Start Master Mix 200 rxns","offer_id":52179873988946,"sku":"PR1001-HFHS-200","price":158.35,"currency_code":"USD","in_stock":true},{"title":"Accuris High Fidelity Hot Start Master Mix 500 rxns","offer_id":52179874021714,"sku":"PR1001-HFHS-500","price":356.61,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0936\/5651\/4898\/files\/Benchmark-Scientific-Accuris_-High-Fidelity-PCR.webp?v=1764686346","url":"https:\/\/medidepot.com\/products\/benchmark-scientific-accuris-high-fidelity-pcr","provider":"MediDepot","version":"1.0","type":"link"}