Typically 5-7 Business Days
Registered & Certified Medical Equipment
Need help?
Typically 5-7 Business Days
Registered & Certified Medical Equipment
Need help?
NEST Scientific 5-Layer Cell Culture Flask, Plug Seal or Vent Cap, Straight Neck, TC, Sterile 1/pk, 8/cs, 870cm² Growth Area Equivalent to 5 X 175cm² Flasks (731001) or 825cm² Growth Area Equivalent to 5 X 175cm² Flasks (731002), 30-50mL per Layer Recommended Working Volume, Flasks Feature Frosted Writing Areas for Easy Culture Identification
NEST Scientific 5-Layer Cell Culture Flasks provide an advanced, high-yield solution engineered to scale up adherent cell culture production while drastically saving valuable incubator space. Designed to deliver the growth surface equivalent of five standard 175 cm² flasks within a single compact vessel, these multi-layer flasks maximize lab efficiency without expanding your footprint. Constructed from high-purity USP Class VI polystyrene with polyethylene caps, each flask utilizes ultrasonic welding without extractable or leachable adhesives to preserve culture integrity.
The tissue culture (TC) surface treatment via plasma etching promotes optimal adherent cell attachment and uniform growth across all layers. Manufactured in an ISO 13485:2016 and cGMP-compliant cleanroom facility, every unit is electron-beam sterilized to achieve a Sterility Assurance Level (SAL) of 10⁻⁶. Available with either a plug seal cap or a 0.22 µm hydrophobic membrane vent cap for sterile continuous gas exchange, these flasks provide a reliable, certified non-pyrogenic, and animal-component-free platform for demanding biological applications.
Features
- 870cm² growth area equivalent to 5 X 175cm² flasks (731001)
- 825cm² growth area equivalent to 5 X 175cm² flasks (731002)
- TC treated - plasma etched for adherent cells
- Plug seal caps or Vent Caps with hydrophobic 0.22um membrane for sterile gas exchange
- 1 per individually wrapped sterile bag, 8 flasks per case
- 30-50mL per layer recommended working volume
- Sterile
Product Details
- Flasks feature frosted writing areas for easy culture identification
- Each flask has a clear lot number printed on each flask for traceability
- Ultrasonically welded layers, no extractable or leachable adhesives used
- Flasks use USP grade VI polystyrene (flask) and polyethylene (caps)
Quality Details
- Manufactured in cGMP (21 CFR part 820/821) compliant factory
- Produced in ISO 13485:2016 and ISO 9001 facility
- Molded and packed in ISO 7 cleanroom
- E-beam sterilized and lot tested to SAL 10^-6
- Lot tested free of pyrogens USP 85
- Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
- Lot tested free DNase/RNase and DNA
- Lot tested free BSE/TSE
- Certified free of animal products
Product Specifications
| Specification | Details |
| Catalog / SKU Numbers | 731001 (Plug Seal Cap), 731002 (Vent Cap) |
| Growth Area (Cat. No. 731001) | 870 cm² |
| Growth Area (Cat. No. 731002) | 825 cm² |
| Neck Type | Straight Neck |
| Cap Options | Plug Seal Cap (731001), 0.22 µm Vent Cap (731002) |
| Surface Treatment | TC Treated (Plasma Etched) |
| Recommended Working Volume | 30–50 mL per layer (150–250 mL total) |
| Flask Material | USP Grade VI Polystyrene |
| Cap Material | Polyethylene |
| Assembly Method | Ultrasonic Welding (No Adhesives) |
| Sterilization Method | E-Beam Sterilization (SAL 10⁻⁶) |
| Cleanroom Standard | ISO 7 Cleanroom Molded and Packed |
| Biological Certifications | Non-Pyrogenic (USP 85), Non-Cytotoxic (USP 87/88) |
| Purity Standards | DNase/RNase Free, DNA Free, BSE/TSE Free |
| Animal Origin | Certified Free of Animal Products |
| Quality Certifications | ISO 13485:2016, ISO 9001, cGMP (21 CFR Part 820/821) |
| Packaging Configuration | 1 per Individually Wrapped Sterile Bag, 8 Flasks per Case |
Standard delivery typically takes 5-7 business days
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